Naegleria fowleri Carter
30894 ™
- Product category
-
Protists
- Product type
-
Parasitic protozoan
- Strain designation
- Lee (L.L.)
- Type strain
- No
- Isolation source
- Cerebrospinal fluid
- Geographical isolation
- United States; Virginia; Richmond
- Product format
- Frozen
- Storage conditions
-
-80°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Documentation
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Required Products
These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.
Detailed product information
Characteristics
- Comments
-
Arginine-dependent cytolytic mechanicmResistence to complement-mediated lysisInnate resistance of miceImmunization of miceAlterations in protein expression and complement resistanceVarying the serum component in axenic cultivationDNA fingerprintingbiochemical identificationMulticomponent hemolytic systemMembrane vesiculation for resisting complement damageModulation of virulence by alterations in growth mediaInterrepeat PCRTransmission between miceSubcellular distribution of hydrolasesThermal ecologySerum agglutination and immunoglobulin levels in infected miceInfection acquired by mice through swimmingCytopathogenicityComparison of two species cultivated in the same nutrient mediumMethod for assessing the migratory responseDifferentiation of Naegleria fowleri from Acanthamoeba using monocolonal antibody
Handling information
- Medium
- Instruction for complete medium
-
Media: ATCC Medium 1034 (ATCC Medium 1034 is available in a freeze-dried format as ATCC® Cat. No. 327-X)
Alternate Media: ATCC Medium 710, ATCC Medium 803, ATCC Medium 902 - Temperature
-
35°C
- Culture system
- Axenic
- Handling procedure
-
Storage and Culture Initiation
Frozen ampules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.- To thaw a frozen ampule, place it in a 35°C water bath , until thawed (2-3 min). Immerse the ampule t a level just sufficient to cover only the frozen material. Do not agitate the ampule.
- Immediately after thawing, aseptically transfer contents to a plastic screw-capped tube containing 5 mL ATCC Medium 1034. Incubate the tube on a 15° horizontal at 35°C with the cap screwed on tightly.
- Culture maintenance
-
- Vigorously agitate a culture at or near peak density and aseptically transfer 0.1-0.2 mL to a fresh tube of ATCC medium 1034.
- Incubate upright at 35°C with the caps on tightly.
- Cryopreservation
-
- Harvest cells from a culture that is at or near peak density by centrifugation at 600 x g for 5 min. Pool the cell pellets into a single tube.
- Adjust the concentration of cells to 2.0 x 106/mL. If the concentration is too low, centrifuge at 600 x g for 5 minutes and resuspend the cell pellet with a volume of supernatant to yield the desired concentration.
- Prepare a 15% (v/v) sterile DMSO solution in ATCC medium 1034 as follows: Add the required volume of DMSO to a glass screw-capped test tube and place on ice. Allow the DMSO to solidify. Add the required volume of refrigerated ATCC medium 1034. Dissolve the DMSO by inverting several times. If the DMSO solution is not prepared on ice, an exothermic reaction will occur that may precipitate certain components of the medium.
- Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 106 and 7.5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no less than 15 min and no longer than 60 min.
- Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
- Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge the ampules into liquid nitrogen.
- The frozen preparations are stored in either the vapor or liquid phase of a nitrogen refrigerator.
- To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial enough to cover only the frozen material. Do not agitate the vial.
- Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate into 5.0 mL of fresh ATCC medium 1034.
- Incubate the tube on a 15° horizontal at 35°C with the cap screwed on tightly.
History
- Deposited as
- Naegleria fowleri Carter
- Depositors
- DT John
- Chain of custody
- ATCC <-- DT John <-- E.C. Nelson
- Type of isolate
- Human
- Year of origin
- 1968
- Patient age
- 15 years
- Patient gender
- Female
- Special collection
-
NCRR Contract
Legal disclaimers
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
- Warranty
-
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
- Disclaimers
-
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
Permits & Restrictions
Import Permit for the State of Hawaii
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Frequently Asked Questions
References
Curated Citations
Haggerty RM, John DT. Innate resistance of mice to experimental infection with Naegleria fowleri. Infect. Immun. 20: 73-77, 1978. PubMed: 669800
John DT, et al. Immunization of mice against Naegleria fowleri infection. Infect. Immun. 16: 817-820, 1977. PubMed: 892900
Daggett PM, Nerad TA. The biochemical identification of vahlkampfiid amoebae. J. Protozool. 30: 126-128, 1983. PubMed: 6864593
Marciano-Cabral F, et al. Cytopathic action of Naegleria fowleri amoebae on rat neuroblastoma target cells. J. Protozool. 37: 138-144, 1990. PubMed: 2108243
Haight JB, John DT. Varying the serum component in axenic cultures of Naegleria fowleri. Proc. Helminthol. Soc. Wash. 49: 127-134, 1982.
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