Pseudomonas carboxydohydrogena (Sanjieva and Zavarzin) Meyer et al.
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #3 agar slant and/or plate.
5. Incubate the tubes and plate at 26oC for 10 to 14 days.
1.0 mm in diameter. The under side of the colony is slightly adherent. This strain will also grow on Brain Heart Infusion (ATCC Medium #44) and Trypticase Soy (ATCC Medium #18). On these media, colonies are entire, smooth, glistening, high convex, opaque, and measure 0.5 to 2.0 mm in diameter. They are not adherent. Strain may grow better in these media, but the typical rosette morphology is better seen on Medium #36. Also, growth is generally better on agar than broth for all media.
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Meyer O, et al. Pseudomonas carboxydohydrogena (Sanjieva and Zavarzin) comb. nov., a monotrichous, nonbudding, strictly aerobic, carbon monoxide-utilizing hydrogen bacterium previously assigned to Seliberia. Int. J. Syst. Bacteriol. 30: 189-195, 1980.
Sanzhieva EU, Zavarzin GA. Bacteria oxidizing carbon monoxide. Dokl. Akad. Nauk SSSR 196: 956-958, 1971. PubMed: 5576296
. . Mikrobiologiya 41: 813-817, 1972.
Mikrobiologiya 41: 936-946, 1972.