Actinoplanes ferrugineus Palleroni
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2. Using a single tube of #1877 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth, a slant, and/or plate.
5. Incubate the tubes and plate at 26°C for 6-10 days.
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Palleroni NJ. New species of the genus Actinoplanes, Actinoplanes ferrugineus. Int. J. Syst. Bacteriol. 29: 51-55, 1979.
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.