Brachyspira innocens (Kinyon and Harris) Ochiai et al.
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Anaerobic conditions for transfer may be obtained by the use of an anaerobic gas chamber or placement of test tubes under a gassing cannula system connected to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
Anaerobe Systems Brucella Blood plate (AS-111 or AS-141) can be used to analyze colony morphology and purity.
Colonies on Brucella agar are thin layer of growth with β- hemolysis seen.
Always use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions. Resazurin in the media is a color indicator for anaerobic conditions. Observance of pink color in medium before use or during incubation shows anaerobic conditions has not been met and oxidation has occurred. Medium should be discarded.
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Stanton TB, et al. Reclassification of Treponema hyodysenteriae and Treponema innocens in a new genus, Serpula gen. nov., as Serpula hyodysenteriae comb. nov. and Serpula innocens comb. nov.. Int. J. Syst. Bacteriol. 41: 50-58, 1991. PubMed: 1704792
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.
Validation list no. 64. Int. J. Syst. Bacteriol. 48: 327-328, 1998.
Ochiai S, et al. Unification of the genera Serpulina and Brachyspira, and proposal of Brachyspira hyodysenteriae comb nov., Brachyspira innocens comb. nov. and Brachyspira pilosicoli comb. nov.. Microbiol. Immunol. 41: 445-452, 1997. PubMed: 9251055