Streptococcus pleomorphus Barnes et al.
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2. Under anaerobic conditions, withdraw 0.5 ml of recommended broth from a single test tube (5 to 6 ml) and rehydrate the entire vial contents.
3. Aseptically transfer this aliquot back into the broth tube. A slant and a pre-reduced blood plate may also be inoculated with 0.1 ml each of the cell suspension. An aerobic blood plate may also be streaked to check for purity.
4. Incubate tubes and one plate under anaerobic conditions at 37°C. Incubate one blood plate aerobically at 37°C.
5. Within 48 hours, growth is evident by turbidity in the broth. On agar surfaces, colonies are gray, flat or raised, glistening, and undulate Further incubation at room temperature produces weak beta-hemolysis. No growth should occur on the blood agar plate incubated aerobically.
Anaerobic conditions for transfer may be obtained by either of the following:
· Use of an anaerobic gas chamber, or
· Placement of test tubes under a gassing cannula system hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
· Loose screw caps on test tubes in anaerobic chamber,
· Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
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Barnes EM, et al. Steptococcus pleomorphus sp. nov.: an anaerobic streptococcus isolated mainly from the caeca of birds. J. Gen. Microbiol. 102: 45-53, 1977.
Validation list no. 2. Int. J. Syst. Bacteriol. 29: 79-80, 1979.
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.