Micromonospora rosaria (Wagman et al.) Horan and Brodsky
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2. From a single tube of #172 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the pellet.
3. Transfer the rehydrated pellet back into the broth tube. Mix well.
4. Use several drops of this suspension to inoculate a second tube of broth, a slant and/or a plate.
5. Incubate all tubes and plates at 26oC for 7 to 14 days
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Lee BK, et al. A method for the biosynthetic preparation of [methyl-14] rosamicin. J. Appl. Bacteriol. 40: 217-221, 1976. PubMed: 1270370
Wagman GH, et al. A new Micromonospora-produced macrolide antibiotic, rosamicin. J. Antibiot. 25: 641-646, 1972. PubMed: 4647834
Horan AC, Brodsky BC. Micromonospora rosaria sp.nov., nom. rev., the Rosaramicin producer. Int. J. Syst. Bacteriol. 36: 478-480, 1986.