Haemophilus paraphrohaemolyticus Zinnemann et al.
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2. Using a single tube of #5129 (5 to 6 ml), withdraw 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet. Aseptically transfer this aliquot back into the broth tube. Mix well.
3. Aseptically transfer 0.2 ml of this suspension to additional #5129 broth tubes, #814 slant(s) and/or 0.1 ml to plates.
4. Incubate tubes and plates at 37oC in an atmosphere of 5% CO2.
5. Within 24 to 48 hours, growth is evident by colonies on the agar surfaces.
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Zinnemann K, et al. A haemolytic V-dependent CO2-preferring Haemophilus species (Haemophilus paraphrohaemolyticus nov. spec.). J. Med. Microbiol. 4: 139-143, 1970. PubMed: 5548598
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.