Burkholderia glathei (Zolg and Ottow) Vandamme et al.
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2. From a single tube of #44 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Use several drops of this suspension to inoculate a second tube of broth, a slant, and/or a plate.
5. Incubate all tubes and plate at 30°C for 48 hours.
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Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.
Vandamme P, et al. Occurrence of multiple genomovars of Burkholderia cepacia in cystic fibrosis patients and proposal of Burkholderia multivorans sp. nov.. Int. J. Syst. Bacteriol. 47: 1188-1200, 1997. PubMed: 9336927
Zolg W, Ottow JC. Pseudomonas glathei sp. nov., a new nitrogen-scavening rod isolated from acid lateritic relicts in Germany. J. Comp. Neurol. 164: 287-299, 1975. PubMed: 1176650