Flavobacterium flevense (van der Meulen et al.) Bernardet et al.
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2. From a tube of #793 broth (5 to 6 ml), withdraw approximately 0.5 with a sterile pipette; add this medium to the vial to rehydrate the entire pellet.
3. Aseptically transfer the contents of the vial into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #793 agar slant and/or plate.
5. Incubate all tubes and plate at 26°C for 48 to 72 hours.
Colonies at 48 to 72 hours are entire, smooth, glistening, low convex, and pale yellow. Pigment deepens to gold at 9 nine days. Luxurious growth will also occur on Tryptic Soy Agar, but the medium above was that recommended by the depositor. On some media, two colony types may be observed, but were identical in the ability to digest agar.
Cells are rods in singles, clumps, and chains. As the cells age, they swell, form sphaeroplasts, and lyse rapidly.
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van der Meulen HJ, Harder W. Characterization of the neoagarotetra-ase and neoagarobiase of Cytophaga flevensis. Antonie van Leeuwenhoek 42: 81-94, 1976. PubMed: 8001
van der Meulen HJ, et al. Isolation and characterization of Cytophaga flevensis sp. nov., a new agarolytic flexibacterium. Antonie van Leeuwenhoek 40: 329-346, 1974. PubMed: 4604642
Bernardet JF, et al. Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int. J. Syst. Bacteriol. 46: 128-148, 1996.
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.
Van Der Meulen HJ, Harder W. Production and characterization of the agarase of Cytophaga flevensis. Antonie van Leeuwenhoek 41: 431-447, 1975. PubMed: 1083206