Micromonospora echinospora Luedemann and Brodsky
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2. Using a single tube of #172 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #172 agar slant and/or plate.
5. Incubate the tubes and plate at 26°C for 7-14 days.
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Wagman GH, et al. Antibiotic G-418, a new Micromonospora-produced aminoglycoside with activity against protozoa and helminths: Fermentation, isolation, and preliminary characterization. Antimicrob. Agents Chemother. 6: 144-149, 1974.
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.