Streptomyces cavourensis subsp. washingtonensis Skarbek and Brady
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2. Using a single tube of ISP #1877 broth (5 to 6 ml), withdraw approximately 0.5 ml to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube and mix well.
4. Use several drops of the cell suspension to inoculate a second tube of broth, a slant and/or a plate.
5. Incubate all tubes and plate at 26°C for 48 hours.
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Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.
Skarbek JD, Brady LR. Isolation of chromomycin A from a new subspecies of Streptomyces. Lloydia 38: 369-377, 1975. PubMed: 811943
Skarbek JD, Brady LR. Streptomyces cavourensis sp. nov. (nom. rev.) and Streptomyces cavourensis subsp. washingtonensis subsp. nov., a chromomycin-producing subspecies. Int. J. Syst. Bacteriol. 28: 45-53, 1978.