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Ureaplasma urealyticum

27618

This is a bacterial type strain isolated from a patient with nongonococcal urethritis. This strain produces restriction endonuclease Uur960I.
Product category
Bacteria
Strain designation
T-strain 960 (CX8) [960, CIP 103755, NCTC 10177]
Type strain
Yes
Isolation source
Nongonococcal urethritis
Product format
Frozen
Storage conditions
-80°C or colder
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Produces restriction endonuclease Uur960I
Preceptrol
No

Characteristics

Serotype
VIII

Handling information

Medium
Temperature
37°C
Atmosphere
Broth: Aerobic
Plates: Anaerobic
Handling procedure
  1. Follow instructions as suggested for the culturing of Mollicutes:
PROCEDURES FOR PROPAGATING MOLLICUTES:
    1. Open the thawed vial according to the enclosed instructions.
    2. Make serial dilutions by transferring the entire contents of the vial to a test tube containing 4.5 mL of appropriate broth. Repeat process by transferring 0.5 mL from the second to third tube, etc.
    3. Use an uninoculated tube of broth to serve as a control.
    4. Plates may be inoculated to check colony morphology. You can also spot each dilution on the surface of plate to determine the number of colony-forming units. However, not all strains do well on solid medium.
    5. Incubate all tubes and plates under the recommended conditions and appropriate temperature (see step 2). The time necessary for growth will vary from strain to strain. Growth in broth for this strain will generally be observed at 16 to 72 hours. Growth on plates generally requires 6 to 10 days.
    6. Depending on the medium used, growth will be indicated by increased turbidity, a color change, or both (see notes section).
    7. Inoculate a Trypticase Soy Agar with 5% Defibrinated Sheep Blood plate with 0.1 mL to check for contamination. Incubate plate at 37°C. A second plate may be incubated in the jar with the growth plates. No growth should occur on Trypticase Soy Agar with 5% Defibrinated Sheep Blood.
  1. Tubes may be incubated aerobically, but plates require increased CO2 (10% or higher) for growth. This is ideally achieved using an anaerobe jar with gas generating sachets. The incubation temperature is 37°C.
Handling notes

Ureaplasma strains grow very rapidly. The indicator in the first tube will change color to a dark green within hours. The culture should be transferred when the broth turns a light to medium green. If it is allowed to turn dark green or blue-green, it has become too alkaline. It is especially important to make serial dilutions of this strain, for when alkaline conditions are reached (as indicated by the color change), the culture will rapidly die unless refrigerated immediately (+4°C) or stored frozen at -60°C. Refrigerated broth cultures may remain viable for periods up to 4 days. No visible turbidity will be seen. The color change is the only indication of growth in broth. Therefore, transfer, freeze, or lyophilize the culture as soon as possible. There should be no growth on GM agar (Genital Mycoplasma) medium. Broth is the best method for propagation.

Store vials at freezer temperatures until ready to use.

Additional information on this culture is available on the ATCC® web site at www.atcc.org.

History

Deposited as
Mycoplasma sp.
Depositors
MC Shepard
Chain of custody
ATCC <-- MC Shepard <-- F Black 960 (cloned 5 times) <-- R Purcell 960 (cloned 3 times) <-- N Somerson <-- MC Shepard 960
Cross references
GenBank AF270762 Ureaplasma urealyticum serovar 8 elongation factor Tu (tuf) gene, complete cds.
GenBank AF272625 Ureaplasma urealyticum serovar 8 23S ribosomal RNA and 5S ribosomal RNA genes, complete sequence; and dihydrofolate reductase (folA) gene, partial cds.
GenBank AF272609 Ureaplasma urealyticum serovar 8 23S ribosomal RNA and 5S ribosomal RNA genes, complete sequence; and transcription elongation factor (greA) gene, partial cds.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Cocks BG, Finch LR. Characterization of a restriction endonuclease from Ureaplamsa urealyticum 960 and differences in deoxyribonucleic acid modification of human ureaplasmas. Int. J. Syst. Bacteriol. 37: 451-453, 1987.

Shepard MC, et al. Ureaplasma urealyticum gen. nov., sp. nov.: Proposed nomenclature for the human T (T-strain) Mycoplasmas. Int. J. Syst. Bacteriol. 24: 160-171, 1974.

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

Robertson JA, et al. Proposal of Ureaplasma parvum sp. nov. and emended description of Ureaplasma urealyticum (Shepard et al. 1974) Robertson et al. 2001. Int. J. Syst. Evol. Microbiol. 52: 587-597, 2002. PubMed: 11931172

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