Micromonospora inyonensis Kroppenstedt et al.
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2. Using a single tube of #172 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #172 agar slant and/or plate.
5. Incubate the tubes and plate at 26°C for 3-5 days.
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Salas JA, Cundliffe E. Characterization in Micromonospora inyoensis of aminoglycoside acetyltransferase activity not previously encountered among actinomycetes. J. Gen. Microbiol. 131: 451-457, 1985. PubMed: 4020339