Bacillus halodurans Nielsen et al.
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2. From a single tube of #661 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well
4. Use several drops of this suspension to inoculate a second tube of broth, a slant and/or a plate.
5. Incubate tubes and plate at 37oC for 24-48 hours.
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Validation list no. 55. Int. J. Syst. Bacteriol. 45: 879-880, 1995.
Nielsen P, et al. Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. Microbiology 141: 1745-1761, 1995.
Boyer EW, et al. Bacillus alcalophilus subsp. halodurans subsp. nov., an alkaline-amylase-producing, alkalophilic organism. Int. J. Syst. Bacteriol. 23: 238-242, 1973.