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Genomic DNA from Methylobacterium radiotolerans strain O-1

27329D-5

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Genomic DNA isolated from Methylobacterium radiotolerans strain O-1. This bacterial strain is also available as ATCC® Catalog No. 27329™.
Product category
Bacteria
Product type
Nucleic acid
Derived from
Methylobacterium radiotolerans O-1 [IAM 12099, NCIB 10815] (ATCC 27329)
Organism
Methylobacterium radiotolerans (Ito and Iizuka) Green and Bousfield
Type strain
Yes
Genome sequenced strain
Yes
Applications
Bioinformatics
Next-generation sequencing
Mass
5 μg
Product format
Freeze-dried
Shipping information
Stored in 1X TE buffer
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

Characteristics

Comments
This preparation of high molecular weight DNA is appropriate for the use in the polymerase chain reaction (PCR)* process and other molecular biology applications.
*the PCR process is covered by patents owned by Hoffmann-La Roche Inc. Use of the PCR process requires a license.
Genomic DNA from genome sequenced strain

Handling information

Handling procedure
  1. Thaw the vial at room temperature and immediately place on ice. Avoid exposing the DNA to repeated freeze-thaw cycles as it may result in degradation of the DNA.
  2. Gently mix the sample to ensure an even distribution of material.
  3. Briefly centrifuge the tube before opening to ensure all liquid is at the bottom.
Handling notes
This preparation of high molecular weight DNA is appropriate for the use in the polymerase chain reaction (PCR)* process and other molecular biology applications.
*the PCR process is covered by patents owned by Hoffmann-La Roche Inc. Use of the PCR process requires a license.

Quality control specifications

Total amount
Total DNA by PicoGreen® measurement was found to be approximately 5 μg.
Integrity
Integrity of DNA was determined by electrophoresis on a 1% agarose gel stained with SYBR Safe™, and was found to be of high molecular weight.
Functional tests
Functional activity was confirmed by PCR amplification of the 16S ribosomal RNA gene.
Identity
Identity confirmed by sequencing of 16S ribosomal RNA gene (first ~500 base pairs).
Verification method
Whole-genome Sequencing

History

Depositors
ATCC
Cross references
GenBank CP001001 Methylobacterium radiotolerans JCM 2831, complete genome.
GenBank CP001002 Methylobacterium radiotolerans JCM 2831 plasmid pMRAD01, complete sequence.
GenBank CP001003 Methylobacterium radiotolerans JCM 2831 plasmid pMRAD02, complete sequence
GenBank CP001004 Methylobacterium radiotolerans JCM 2831 plasmid pMRAD03, complete sequence.
GenBank CP001005 Methylobacterium radiotolerans JCM 2831 plasmid pMRAD04, complete sequence.
GenBank CP001006 Methylobacterium radiotolerans JCM 2831 plasmid pMRAD05, complete sequence.
GenBank CP001007 Methylobacterium radiotolerans JCM 2831 plasmid pMRAD06, complete sequence.
GenBank CP001008 Methylobacterium radiotolerans JCM 2831 plasmid pMRAD07, complete sequence.
GenBank CP001009 Methylobacterium radiotolerans JCM 2831 plasmid pMRAD08, complete sequence.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Frequently Asked Questions

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