Mycobacterium aichiense (Tsukamura) Tsukamura
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2. Using a single tube of #1395 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #173 or #90 agar slant and a #173 plate.
5. Incubate the tubes and plate at 37oC for 7 days.
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Tsukamura M. New species of rapidly growing scotochromogenic mycobacteria, Mycobacterium chubuense Tsukamura, Mycobacterium aichiense, Tsukamura, and Mycobacterium tokaiense Tsukamura. Med. Biol. 86: 13-17, 1973.
Tsukamura M, et al. Numerical analysis of rapidly growing, scotochromogenic mycobacteria, including Mycobacterium obuense sp. nov., nom. rev., Mycobacterium rhodesiae sp. nov., nom. rev., Mycobacterium aichiense sp. nov., nom. rev., Mycobacterium chubuense sp. nov., nom. rev., and Mycobacterium tokaiense sp. nov., nom. rev.. Int. J. Syst. Bacteriol. 31: 263-275, 1981.