Marinobacterium stanieri (Baumann et al.) Satomi et al.
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2. Using a single tube of #2 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #2 agar slant and/or plate.
5. Incubate the tubes and plate at 26°C for 24-36 hours.
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Baumann P, et al. Taxonomy of marine Pseudomonas species: P. stanieri sp. nov.; P. perfectomarina sp. nov., nom. rev.; P. nautica; and P. doudoroffii. Int. J. Syst. Bacteriol. 33: 857-865, 1983.
. . J. Bacteriol. 110: 423-424, 1972.
Satomi M, et al. Phylogenetic study of the genus Oceanospirillum based on 16S rRNA and gyrB genes: emended description of the genus Oceanospirillum, description of Pseudospirillum gen. nov., Oceanobacter gen. nov. and Terasakiella gen. nov. and transfer of Oceanospirillum jannaschii and Pseudomonas stanieri to Marinobacterium as Marinobacterium jannaschii comb. nov. and Marinobacterium stanieri comb. nov.. Int. J. Syst. Evol. Microbiol. 52: 739-747, 2002. PubMed: 12054233