• Quick Order

Bdellovibrio sp.


Product category
Strain designation
ICPB 3326 [Bd. W]
Type strain
Product format
Storage conditions
2°C to 8°C
Buy Now
Price: $567.00 EA
Discounts may be available for our fellow nonprofit organizations.

Generally ships within 1-3 business days


ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information


Specific applications
Produces encysted resting stages


Predator of Rhodospirillum rubrum (ATCC® 27048™)

Handling information

Handling procedure
ATCC® 27047™ is a predator of ATCC® 27048™ Rhodospirillum rubrum and is host-dependent.  Therefore, it is necessary to establish a growing culture of the host strain before opening the vial of the Bdellovibrio.

Growth of host ATCC® 27048™:
  1. Fill 13 mm screw cap test tubes completely to the top with #550 broth and then add 0.02 mL of 3% cysteine.
  2. Let the test tubes sit for at least one hour prior to opening.
  3. Open vial according to enclosed instructions.
  4. Using a single tube of #550 reduced broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or mL pipette. Rehydrate the entire pellet.
  5. Aseptically transfer this aliquot back into the broth tube. Mix well.
  6. Use 0.5 mL of the suspension to inoculate a #550 secondary broth tube.
  7. Incubate the tubes at 30°C for 3 to 7 days under a tungsten lamp. Ensure that the screw cap tops are sealed during the incubation period.

Propagation of Bdellovibrio:
  1. Open a vial of ATCC® 27047™ according to enclosed instructions.
  2. Rehydrate the entire pellet with 0.5 mL of #587 broth. Withdraw suspension into a sterile test-tube.
  3. Add approximately 0.5 mL of the host strain.             
  4. Melt tubes containing 2.5 mL of 0.8% semi-solid agar, and place in a 45°C water bath.
  5. Add 0.2 to 0.3 mL of the host/predator suspension to each melted tube and pour over the surface of pre-warmed #587 plates.
  6. Incubate plates anaerobically at 30°C for at least 10 days.  After this time, plaques may be visible.  Observe daily until plaques or clearing are seen. 
  7. Scrape off overlay into a test-tube and centrifuge at low speed to sediment the agar and cellular debris.  The Bdellovibrio cells should be in the supernatant. Check for tiny, rapidly moving cells under a wet mount.
Handling notes
This item will exhibit growth under aerobic conditions but growth is better under anaerobic conditions.
When further propagating the strain, use the tube of the host set aside for this purpose to obtain a more concentrated suspension of the host.  It should be transferred and used as a 24 hour culture.
Additional information on this culture is available on the ATCC® web site at


Deposited as
Bdellovibrio sp.
MP Starr
Chain of custody
ATCC <-- MP Starr <-- G. Drews strain Bd. W
Cross references
GenBank M61238 Bdellovibrio sp. 16S rRNA gene, partial cds.
GenBank AJ292518 Bdellovibrio sp. W partial 16S rRNA gene, strain ATCC 27047.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.



Curated Citations

Burger A, et al. Host range and infection cycle of a newly isolated strain of Bdellovibrio bacteriovorus. Arch. Mikrobiol. 61: 261-279, 1968. PubMed: 4974080

Frequently Asked Questions

Need assistance with this product? Contact our Technical Support team.



US and Puerto Rico


Outside the US


Hours of Operation

9:30am - 5:30pm
US Eastern Time