Bacillus licheniformis (Weigmann) Chester
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2. Using a single tube of #18 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #18 agar slant and/or plate.
5. Incubate the tubes and plate at 30oC for 24 hours
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Yudkin MD. Protein synthesis by long-lived messenger ribonucleic acid in bacteria. Biochem. J. 100: 501-506, 1966. PubMed: 4961300