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Genomic DNA from Escherichia coli strain FDA strain Seattle 1946

25922D-5

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Genomic DNA from Escherichia coli FDA strain Seattle 1946 (ATCC 25922). This whole-genome sequenced product can be used in PCR and other molecular biology applications. 
Product category
Bacteria
Product type
Nucleic acid
Derived from
Escherichia coli FDA strain Seattle 1946 [DSM 1103, NCIB 12210] (ATCC 25922)
Organism
Escherichia coli (Migula) Castellani and Chalmers
Type strain
No
Genome sequenced strain
Yes
Applications
Bioinformatics
Food testing
Next-generation sequencing
Mass
5 μg
Product format
Dried
Shipping information
Stored in 1X TE buffer
Storage conditions
2°C to 8°C

Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
Food testing

Characteristics

Antigenic properties
Biotype 1
Serotype
O6
Comments
This preparation of high molecular weight DNA is appropriate for use in the polymerase chain reaction (PCR)* process and other molecular biology applications.
*the PCR process is covered by patents owned by Hoffmann-La Roche Inc. Use of the PCR process requires a license.

Handling information

Handling procedure
Centrifuge tube prior to opening to prevent loss of pelleted material
  1. Rehydrate contents of vial with molecular grade H2O.
  2. Place vial at 37°C for 1 hour or at 2°C to 8°C overnight.
  3. For more complete rehydration and to fully recover DNA, incubate the sample overnight at 4°C while rocking; then incubate for 1 hour at 65°C.  Resuspending the dried DNA in ≥250 µL may give better results.
Handling notes
Genomic DNA isolated from bacteria is appropriate for PCR* and other molecular biology applications.
*The polymerase chain reaction (PCR) process is covered by patents owned by Hoffmann-LaRoche Inc. Use of the PCR process requires a license.

Quality control specifications

Electrophoresis - RNA content
No RNA was detected by electrophoresis
Total amount
Total DNA by PicoGreen® measurement was found to be approximately 5 µg.
Integrity
Integrity of DNA was determined by electrophoresis on a 1% agarose gel stained with SYBR Safe™, and was found to be of high molecular weight.
Functional tests
Functional activity was confirmed by PCR amplification of the 16S ribosomal RNA gene.
Identity
Identity confirmed by sequencing of 16S ribosomal RNA gene (first ~500 base pairs).
Verification method
Whole-genome Sequencing

History

Deposited as
Escherichia coli (Migula) Castellani and Chalmers
Depositors
ATCC
Cross references
GenBank AF038431 Escherichia coli DNA gyrase A (gyrA) gene, partial cds.
GenBank AX111547 Sequence 2280 from Patent WO0123604.
GenBank AX110242 Sequence 975 from Patent WO0123604.
GenBank AX110212 Sequence 945 from Patent WO0123604.
GenBank X80724 E. coli (ATCC 25922) gene for 16S rRNA.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Frequently Asked Questions

References

Need assistance with this product? Contact our Technical Support team.

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