Labrenzia aggregata (Uchino et al.) Biebl et al.
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. Using a single tube of #2 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #2 agar slant and/or plate.
5. Incubate the tubes and plate at 26oC for 48 hours.
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Ahrens R. Taxonomische Untersuchungen an sternbildenden Agrobacterium-Arten aus der westlichen Ostsee. Kiel. Meeresforsch. 24: 147-173, 1968.
Ruger HJ, Hofle MG. Marine star-shaped-aggregate-forming bacteria: Agrobacterium atlanticum sp. nov.; Agrobacterium meteori sp. nov.; Agrobacterium ferrugineum sp. nov., nom. rev.; Agrobacterium gelatinovorum sp. nov., nom. rev.; and Agrobacterium stellulatum sp. nov., nom rev.. Int. J. Syst. Bacteriol. 42: 133-143, 1992. PubMed: 1371058
Validation list no. 68. Int. J. Syst. Bacteriol. 49: 1-3, 1999.
Uchino Y, et al. Reclassification of marine Agrobacterium species: proposals of Stappia stellulata gen. nov., comb. nov., Stappia aggregata sp. nov., nom. rev., Ruegeria atlantica gen. nov., comb. nov., Ruegeria gelatinovora comb. nov., Ruegeria algicola comb. nov., and Ahrensia kieliense gen. nov., sp. nov., nom. rev.. J. Gen. Appl. Microbiol. 44: 201-210, 1998. PubMed: 12501429
Baltic Sea sediment