Acholeplasma axanthum Tully and Razin
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
PROCEDURES FOR PROPAGATING MOLLICUTES:
a) Open the vial according to the enclosed instructions.
b) Using a Pasteur or 1.0 ml pipette, withdraw approximately 0.5 to 1.0 ml from a tube containing 5.0 ml. Rehydrate the entire pellet.
c) Aseptically transfer this aliquot back into the tube. Mix well.
d) Make serial dilutions by transferring 0.5 ml from the original tube to a tube containing 4.5 ml. Repeat process by transferring 0.5 ml from the second to a third tube, etc. Dilutions are important, not only for titration purposes, but also to keep culture in varying stages of growth. Many strains will die out rapidly once acid or alkaline conditions are reached. It is recommended to prepare several dilutions from the initial tube as the cryoprotectant used in the freeze‑drying process often inhibits growth.
e) Use an uninoculated tube of broth to serve as a control.
f) Plates may be inoculated to check colonial morphology. You can also spot each dilution on the surface of plate (4 or more/plate) to determine the number of colony-forming units. However, not all strains do well on solid medium.
g) Incubate all tubes and plates under the recommended conditions and appropriate temperature. The time necessary for growth will vary from strain to strain. Growth on plates generally requires additional incubation.
h) Depending on the medium used, growth will be indicated by increased turbidity, a color change, or both.
2. Tubes may be incubated aerobically, but plates are incubated under 5% CO2. The incubation temperature is 37oC.
3. This strain starts to show turbidity in the first few dilution tubes within 24 hours. Additional incubation is required for growth on solid agar.
4. Subsequent, fresh transfers grow more rapidly than the original culture. This strain produces good turbidity.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
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If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Edward DG, Freundt EA. Type strains of species of the order Mycoplasmatales, including designation of neotypes for Mycoplasma mycoides subsp. mycoides, Mycoplasma agalactiae subsp. agalactiae, and Mycoplasma arthritidis. Int. J. Syst. Bacteriol. 23: 55-61, 1973.
Tully JG, Razin S. Characteristics of a new sterol-nonrequiring Mycoplasma. J. Bacteriol. 98: 970-978, 1969. PubMed: 4892385
Tully JG, Razin S. Acholeplasma axanthum, sp. n.: a new sterol-nonrequiring member of the Mycoplasmatales. J. Bacteriol. 103: 751-754, 1970. PubMed: 4919991
Friend C, et al. Antibiotic effect of tylosin on a mycoplasma contaminant in a tissue culture leukemia cell line. Proc. Soc. Exp. Biol. Med. 121: 1009-1010, 1966. PubMed: 4287117
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.