Xanthophyllomyces dendrorhous Golubev
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2. From a single test tube of sterile distilled water (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
3. Aseptically transfer the suspension back into the test tube of sterile distilled water.
4. Let the test tube sit at room temperature (25° C) undisturbed for at least 2 hours; overnight rehydration is recommended.
5. Mix the suspension well. Use several drops to inoculate appropriate solid or liquid media (see atcc.org for a recommended medium and temperature).
6. Incubate cultures at appropriate temperature. Remaining suspension may be stored in refrigerator for possible reuse, although no survival time can be specified.
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Johnson EA, et al. Processes for in vivo production of astaxanthin and Phaffia rhodozyma yeast of enhanced astaxanthin content. US Patent 5,356,809 dated Oct 18 1994
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Castillo A, Cifuentes V. Presence of double-stranded RNA and virus-like particles in Phaffia rhodozyma. Curr. Genet. 26: 364-368, 1994. PubMed: 7882432
Miller MW, et al. Phaffia, a new yeast genus in the Deuteromycotina (Blastomycetes). Int. J. Syst. Bacteriol. 26: 286-291, 1976.
Johnson EA, Schroeder WA. Antioxidant role of carotenoids in Phaffia rhodozyma. J. Gen. Microbiol. 139: 907-912, 1993.