Streptomyces spheroides Wallick et al.
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. Using a single tube of #1877 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #196 agar slant and/or plate.
5. Incubate the tubes and plate at 26oC for 48 hours.
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Wallick H. Antibiotic and process of producing the same. US Patent 3,049,534 dated Aug 14 1962
Stammer CH, Miller IM. Fermentation process for producing novobiocin. US Patent 3,049,475 dated Aug 14 1962
Miller IM. Fermentation process for producing novobiocin. US Patent 3,049,476 dated Aug 14 1962
Spencer C, Walton E. Process for the production of 3-amino-4,7-dihydroxycoumarin hydrochloride. US Patent 3,049,550 dated Aug 14 1962
. . Bergey's Manual Syst. Bacteriol. 4: 2471, 1989.