Streptomyces phaeochromogenes (Conn) Waksman and Henrici
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2. From a single tube of #1877 broth (5 to 6 ml), withdraw approximately 0.6 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the pellet.
3. Use 0.5ml of this suspension to inoculate a #196 slant and 0.1ml to inoculate #196 plates.
4. Incubate tubes and plates at 26oC, under aerobic conditions, for 7 to 14 days.
5. After 7 to 14 days of incubation, wash cells from the slant and transfer this broth to a new slant and plate. Incubate another 7 to 14 days under aerobic conditions. This second transfer and incubation is necessary for complete removal of the cryoprotectant, which can inhibit growth.
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