Bacillus amyloliquefaciens (Fukumoto) Priest et al.
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2. Using a single tube of #423 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth, a slant, and/or plate.
5. Incubate all tubes and plate at 37°C for 24-48 hours.
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Welker NE, Campbell LL. Unrelatedness of Bacillus amyloliquefaciens and Bacillus subtilis. J. Bacteriol. 94: 1124-1130, 1967. PubMed: 4963774
Roberts RJ. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 13: r165-r200, 1985. PubMed: 2987885