Streptomyces glaucescens (Preobrazhenskaya) Pridham et al.
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2. Using a single tube of #1877 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #196 agar slant and/or plate.
5. Incubate the tubes and plate at 26oC for 1-2 weeks.
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Gottlieb D. Designation of type strains of 47 species of Actinomyces (Streptomyces). Int. J. Syst. Bacteriol. 18: 19-20, 1968.
Shirling EB, Gottlieb D. Cooperative description of type cultures of Streptomyces. II. Species descriptions from first study. Int J Syst Bacteriol 18: 69-189, 1968.
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.