Escherichia coli (Migula) Castellani and Chalmers
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UTI assay development
2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #3 agar slant and/or plate.
5. Incubate the tubes and plate at 37oC for 24 hours
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. . Acta Pathol. Microbiol. Scand. Sect. B Microbiol. 20: 21-44, 1943.