Paenibacillus macquariensis subsp. macquariensis (Marshall and Ohye) Ash et al.
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2. Using a single tube of #470 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #470 agar slant and/or plate.
5. Incubate the tubes and plate at 20oC for 2 to 4 days.
On #470 agar plates, colonies appear irregular, translucent, flat mucoid and white. This strain also grows on Trypticase Soy Agar.
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Marshall BJ, Ohye DF. Bacillus macquariensis n. sp., a psychrotrophic bacterium from sub- Antarctic soil. J. Gen. Microbiol. 44: 41-46, 1966. PubMed: 5966293
Validation list no. 52. Int. J. Syst. Bacteriol. 45: 197-198, 1995.
Ash C, et al. Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Antonie van Leeuwenhoek 64: 253-260, 1993. PubMed: 8085788
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.