Bacillus insolitus Larkin and Stokes
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #3 slant, and/or plate
5. Incubate all tubes and plates at 15oC for four to five days.
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. . Curr. Microbiol. 1: 195-199, 1978.
Larkin JM, Stokes JL. Taxonomy of psychrophilic strains of Bacillus. J. Bacteriol. 94: 889-895, 1967. PubMed: 6051360
Larkin JM, Stokes JL. Isolation of psychrophilic species of Bacillus. J. Bacteriol. 91: 1667-1671, 1966. PubMed: 5937228
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.