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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #3 slant, and/or plate
5. Incubate all tubes and plates at 15oC for four to five days.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
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. . Curr. Microbiol. 1: 195-199, 1978.
Larkin JM, Stokes JL. Taxonomy of psychrophilic strains of Bacillus. J. Bacteriol. 94: 889-895, 1967. PubMed: 6051360
Larkin JM, Stokes JL. Isolation of psychrophilic species of Bacillus. J. Bacteriol. 91: 1667-1671, 1966. PubMed: 5937228
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.