Bacillus psychrosaccharolyticus Patel and Gupta
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #3 agar slant and/or plate.
5. Incubate the tubes and plate at 15°C for 2 to 4 days.
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Validation list no. 28. Int. J. Syst. Bacteriol. 39: 93-94, 1989.
Larkin JM, Stokes JL. Taxonomy of psychrophilic strains of Bacillus. J. Bacteriol. 94: 889-895, 1967. PubMed: 6051360
Larkin JM, Stokes JL. Isolation of psychrophilic species of Bacillus. J. Bacteriol. 91: 1667-1671, 1966. PubMed: 5937228
Priest FG, et al. A numerical classification of the genus Bacillus. J. Gen. Microbiol. 134: 1847-1882, 1988. PubMed: 3246588