Microscilla marina (Pringsheim) Pringsheim
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. Using a single tube of #231 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #231 agar slant and/or plate.
5. Incubate the tubes and plate at 30°C for 96-120 hours.
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Lewin RA. A classification of flexibacteria. J. Gen. Microbiol. 58: 189-206, 1969. PubMed: 5360476
Mandel M, Lewin RA. Deoxyribonucleic acid base composition of flexibacteria. J. Gen. Microbiol. 58: 171-178, 1969. PubMed: 5360474
Colwell RR. Numerical taxonomy of the flexibacteria. J. Gen. Microbiol. 58: 207-215, 1969. PubMed: 5360477
Fager EW. Recurrent group analysis in the classification of flexibacteria. J. Gen. Microbiol. 58: 179-187, 1969. PubMed: 5360475
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.