Actinoplanes deccanensis Parenti et al.
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2. Using a single tube of #1877 or #551 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #551 agar slant and/or plate.
5. Incubate the tubes and plate at 26oC for 7 to 10 days.
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Coronelli C, et al. Lipiarmycin and its preparation. US Patent 3,978,211 dated Aug 31 1976
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.
Parenti F, et al. Lipiarmycin, a new antibiotic from Actinoplanes. I. Description of the producer strain and fermentation studies. J. Antibiot. 28: 247-252, 1975.