Micromonospora olivasterospora Kawamoto et al.
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2. From a single tube of #172 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Use several drops of this suspension to inoculate an additional broth tube, one or two #172 agar slants and/or a plate.
5. Incubate the tubes and plate at 26°C for 7 to 14 days.
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Nara T, et al. Fortimicin B and process for production thereof. US Patent 3,931,400 dated Jan 6 1976
Kawamoto I, et al. Micromonospora olivasterospora sp. nov.. Int. J. Syst. Bacteriol. 33: 107-112, 1983.