Streptomyces hiroshimensis (Shinobu) Witt and Stackebrandt
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2. Using a single tube of #1877 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #196 agar slant and/or plate.
5. Incubate the tubes and plate at 26oC for 5-7 days.
On #196 plates aerial mycelia are abundant and light yellowish-pink in color. Vegetative mycelia are rust colored.
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. . Bergey's Manual Syst. Bacteriol. 4: 2499, 1989.
Locci R, et al. The genus Streptoverticillium. A taxonomic study. G. Microbiol. 17: 1-60, 1969.
Shinobu R. Three new species of Streptomyces forming whirls. Mem. Osaka Univ. Lib. Arts Educ. B 5: 84-93, 1956.
Shirling EB, Gottlieb D. Cooperative description of type cultures of Streptomyces. II. Species descriptions from first study. Int J Syst Bacteriol 18: 69-189, 1968.
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.