Streptomyces cinnamoneus (Benedict et al.) Witt and Stackebrandt
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. From a single tube of #1877 broth (5 to 6 ml), withdraw approximately 0.6 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3. Use 0.5ml of this suspension to inoculate a #196 slant and 0.1ml to inoculate #196 plates.
4. Incubate tubes and plates at 26oC, under aerobic conditions, for 14-16 days.
5. After 14-16 days of incubation, wash cells from the slant and transfer this broth to a new slant and plate. Incubate another 14-16 days under aerobic conditions. This second transfer and incubation is necessary for complete removal of the cryoprotectant, which can inhibit growth.
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Hamagishi Y, et al. Nucleotide pyrophosphotransferase and method of preparation. US Patent 4,152,209 dated May 1 1979
Takeuchi T, et al. Process for microbial production of an optically active 3-(3,4-dihydroxyphenyl) serine. US Patent 4,699,879 dated Oct 13 1987
Locci R, et al. The genus Streptoverticillium. A taxonomic study. G. Microbiol. 17: 1-60, 1969.
Yamaguchi T. Studies on the antibiotic substance-producing strains H-2075, H-2069 (S. hachijoensis nov. sp.) and H-3030. J. Antibiot. Ser. A 7: 10-14, 1954.