Mycobacterium parafortuitum Tsukamura et al.
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2. From a single tube of the recommended broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or
1.0 ml pipette and use to rehydrate the pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Heavily inoculate a slant of #90 and/or a plate of #173 agar.
5. After growth has been achieved, additional transfers may be made.
Colonies on #173 plates are tiny, entire, circular, smooth, convex, and buff. This strain also grows on #44, Brain Heart Infusion Broth and Agar.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
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Tsukamura M. Adansonian classification of mycobacteria. J. Gen. Microbiol. 45: 253-273, 1966. PubMed: 4961417
Tsukamura M. Mycobacterium parafortuitum: a new species. J. Gen. Microbiol. 42: 7-12, 1966. PubMed: 5922300
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.