Pseudomonas aeruginosa (Schroeter) Migula
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UTI assay development
2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate an additional broth tube, a #3 agar slant and/or a plate.
5. Incubate all tubes and plate at 37°C for 24 hours.
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Yabuuchi E, Ohyama A. Characterization of "pyomelanin" -- producing strains of Pseudomonas aeruginosa. Int. J. Syst. Bacteriol. 22: 53-64, 1972.
Pharm. J. 203: 529-534, 1969.
Method for estimation of concentration of disinfectants used in dirty conditions in hospitals by the modified Kelsey-Sykes test. London, UK:British Standards Institution;British Standard BS 6905:1987.