Pediococcus parvulus Gunther and White
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2. Using a single tube of #416 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #416 agar slant and/or plate.
5. Incubate the tubes and plate at 30oC for 24-48 hours.
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Gunther HL, White HR. The cultural and physiological characters of the Pediococci. J. Gen. Microbiol. 26: 185-197, 1961. PubMed: 13902978
Int. Bull. Bacteriol. Nomencl. Taxon. 12: 189-190, 1962.
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.