Azotobacter beijerinckii Lipman
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2. From a single tube of #14 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Incubate at 30oC. Growth should occur in 3 to 5 days.
5. After growth appears in the broth, a second tube of broth, a slant, and/or a plate may be inoculated. It is advisable to establish good growth in the primary tube before making subsequent transfers.
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Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.