Rhodococcus rhodochrous (Zopf) Tsukamura emend. Rainey et al.
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2. Using a single tube of #1877 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or glass pipette. Rehydrate the pellet.
3. Transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth, one or two slants and/or a plate.
5. Incubate all tubes and plate at 26oC for three to five days
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Raymond RL, Jamison VW. Microbiological oxidation of alkylbenzenes. US Patent 3,383,289 dated May 14 1968