Derxia gummosa Jensen et al.
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. Using a single tube of #165 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #165 agar slant and/or plate.
5. Incubate the tubes and plate in 70% N2 and 30% air at 30°C for 4-7 days.
Aerobic growth on nutrient medium occurs in 4 days. Good growth in air is obtained only when nitrogen is present in the medium.
It is recommended, however, that this strain is grown in 70% N2 and 30% air on #165 medium. This reduced oxygen tension will help assure a functioning nitrogen-fixing enzyme system.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
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Jennsen HL, et al. A new nitrogen-fixing bacterium: Derxia gummosa nov. gen. nov. spec.. Arch. Mikrobiol. 36: 182-195, 1960. PubMed: 14407096
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.