Pseudonocardia saturnea (Hirsch) Warwick et al.
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2. Using a single tube of #86 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #86 agar slant and/or plate.
5. Incubate the tubes and plate at 26°C for 5-7 days.
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Hirsch P. Some additional Actinomycetes existing on air impurities and their classification. Arch. Mikrobiol. 35: 391-414, 1960. PubMed: 14401988