Actinobacillus seminis (Baynes and Simmons) Sneath and Stevens
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2. Using a single tube of #44 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a second #260 broth, #260 agar slant and/or plate.
5. Incubate the tubes and plate at 37oC for 24 hours.
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Sneath PH, Stevens M. Actinobacillus rossii sp. nov., Actinobacillus seminis sp. nov., nom. rev., Pasteurella bettii sp. nov., Pasteurella lymphangitidis sp. nov., Pasteurella mairi sp. nov., and Pasteurella trehalosi sp. nov.. Int. J. Syst. Bacteriol. 40: 148-153, 1990. PubMed: 2223608
Baynes ID, Simmons GC. Ovine epididymitis caused by Actinobacillus seminis n. sp.. Australes. Vet. J. 36: 454-459, 1960.