Comamonas testosteroni (Talalay) Tamaoka et al. emend. Willems et al.
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #3 agar slant and/or plate.
5. Incubate the tubes and plate at 26oC for 24 hours.
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Stanier RY, et al. The aerobic pseudomonads: a taxonomic study. J. Gen. Microbiol. 43: 159-271, 1966. PubMed: 5963505