Arthrobacter nicotianae Giovannozzi-Sermanni
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate an additional broth tube, a #3 agar slant and/or a plate.
5. Incubate all tubes and plate at 26°C for 24 hours.
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Giovannozzi-Sermanni G. Una nova species di Arthrobacter determinante la degradazione della nicotinea: Arthrobacter nicotinae. Il Tabacco 63: 83-86, 1959.
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.
Roberts RJList of known restriction endonucleasesIn: Roberts RJNew England Biolabs Catalog 1986/87pp. 68-81, 1987