Streptomyces castelarensis (Cercós) Kumar and Goodfellow
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2. Using a single tube of #1877 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #196 agar slant and/or plate.
5. Incubate the tubes and plate at 26°C for 4 to 7 days.
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Cercos AP. Streptomyces rutgersensis var. castelarense n. var. Nuevas propiedades de la canfomicina. Rev. Invest. Agric. 8: 263-283, 1954.
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.
Cercos AP. Canfomicina, antibiotico producido por un "Streptomyces". Rev. Argent. Agron. 20: 53-62, 1953.
Kumar Y, Goodfellow M. Five new members of the Streptomyces violaceusniger 16S rRNA gene clade: Streptomyces castelarensis sp. nov., comb. nov., Streptomyces himastatinicus sp. nov., Streptomyces mordarskii sp. nov., Streptomyces rapamycinicus sp. nov. and Streptomyces ruanii sp. nov. Int. J. Syst. Evol. Microbiol. 58: 1369-1378, 2008. PubMed 18523180