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Staphylococcus epidermidis (Winslow and Winslow) Evans

14990

Type strain.  Quality control strain for API products and the DuPont RiboPrinter™ Microbial Characterization System.
Product category
Bacteria
Product type
Preceptrol culture
Strain designation
Fussel [NCTC 11047, R. Hugh 2466]
Type strain
Yes
Isolation source
Nose
Applications
Media testing
Quality control
Product format
Freeze-dried
Storage conditions
2°C to 8°C
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Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Efficacy testing
Inhibition testing
Media testing
Quality control strain
Sterility testing
Testing antimicrobial handwashing formulations
Quality control strain for API products
Preceptrol
Yes

Handling information

Medium
Temperature
37°C
Atmosphere
Aerobic
Handling procedure
  1. Open vial according to enclosed instructions or visit www.atcc.org for instructions.
  2. Rehydrate the entire pellet with approximately 0.5 mL of #3 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #3 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
  3. Use several drops of the primary broth tube to inoculate a #3 plate and/or #3 agar slant.
  4. Incubate at 37°C for 24-48 hours.
Handling notes
Additional information on this culture is available on the ATCC® web site at www.atcc.org.

History

Deposited as
Staphylococcus epidermidis (Winslow and Winslow) Evans
Depositors
American Meat Institute Foundation
Chain of custody
ATCC <-- American Meat Institute Foundation <-- R Deibel
Cross references
GenBank K02688 S.epidermidis 5S rRNA.
GenBank Z48233 S.epidermidis thyF and dfrC genes.
GenBank AX109637 Sequence 370 from Patent WO0123604.
GenBank AX109878 Sequence 611 from Patent WO0123604.
GenBank AX111494 Sequence 2227 from Patent WO0123604.
GenBank AX111515 Sequence 2248 from Patent WO0123604.
GenBank AX111535 Sequence 2268 from Patent WO0123604.
GenBank Z26894 S.epidermidis gene for 16S ribosomal RNA.
GenBank AB015166 Staphylococcus epidermidis gene, partial cds.
GenBank L37605 Staphylococcus epidermidis 16S ribosomal RNA (16S rRNA) gene.
GenBank U02913 Staphylococcus epidermidis ATCC 14990 16S rRNA gene, partial sequence.
GenBank D83363 Staphylococcus epidermidis (strain ATCC 14990T) gene for 16S rRNA, partial sequence.
GenBank AF298800 Staphylococcus epidermidis translation elongation factor Tu (tuf) gene, partial cds.
GenBank AF162863 Staphylococcus epidermidis Pal1 (pal1) gene, partial cds; TagF (tagF) gene, complete cds; and TpgX (tpgX) gene, partial cds.
GenBank AF012132 Staphylococcus epidermidis agr system including response regulator (agrA), histidine kinase (agrC), AgrD (agrD), AgrB (agrB) and delta toxin (hld) genes, complete cds.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Jones D, et al. Identity of Staphylococcus epidermidis. J. Bacteriol. 85: 62-67, 1963. PubMed: 14029448

Gotz F, Schleifer KH. Purification and properties of a fructose-1,6-diphosphate activated L- lactate dehydrogenase from Staphylococcus epidermidis. Arch. Microbiol. 105: 303-312, 1975. PubMed: 242300

Hugh R, Ellis MA. The neotype strain for Staphylococcus epidermidis (Winslow and Winslow 1908), Evans 1916. Int. J. Syst. Bacteriol. 18: 231-239, 1968.

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

quality control for the DuPont RiboPrinter™ Microbial Characterization System

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