Malikia spinosa (Leifson) Spring et al.
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate an additional broth tube, a #3 agar slant and/or a plate.
5. Incubate all tubes and plate at 26°C for 48 hours.
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Leifson E. Pseudomonas spinosa n. sp.. Int. Bull. Bacteriol. Nomencl. Taxon. 12: 89-92, 1962.
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.
Spring S, et al. Malikia granosa gen. nov., sp. nov., a novel polyhydroxyalkanoate- and polyphosphate-accumulating bacterium isolated from activated sludge, and reclassification of Pseudomonas spinosa as Malikia spinosa comb. nov.. Int. J. Syst. Evol. Microbiol. 55: 621-629, 2005. PubMed: 15774634
river water, Du Page, IL