Mycobacterium acapulcensis Bojalil et al.
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2. Using a single tube of #1395 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Heavily inoculate a slant of #90 and/or a plate of #173 agar.
5. Incubate the tubes and plate at 37oC for approximately five days.
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Bojalil LF, et al. Adansonian classification of mycobacteria. J. Gen. Microbiol. 28: 333-346, 1962. PubMed: 13870716
Cerbon J, Bojalil LF. Physiological relationships of rapidly growing mycobacteria. Adansonian classification. J. Gen. Microbiol. 25: 7-15, 1961. PubMed: 13691937